![]() ![]() All assays were performed in the assay buffer (50 mM TRIS, pH 7.5, 20 mM MgCl 2, 0.1 mg/mL BSA, and 0.05 mM DTT). As an additional background control group, the same serial dilutions of FAM-SRC1-A, FAM-SRC1-B, and FAM-SRC1-C were incubated with Tb-anti-GST (5 nM), but with 1.1% DMSO (vehicle) and without GST-hPXR-LBD. The final DMSO concentration was 1.1% for all wells. Serial dilutions of FAM-SRC1-A, FAM-SRC1-B, and FAM-SRC1-C (1:2 dilutions giving 13 concentration levels from 2500 to 0.6 nM) were incubated with Tb-anti-GST (5 nM), GST-hPXR-LBD (5 nM), and either DMSO or T0901317 (10 μM). Wenwei Lin, Taosheng Chen, in Advances in Protein Chemistry and Structural Biology, 2018 3.1 PXR TR-FRET-Binding K d Determinations for the Fluorescent SRC1 Peptides FAM-SRC1-A, FAM-SRC1-B, and FAM-SRC1-C
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